In Dairojo! Samurai Defenders, players must strategically position various units such as spears, archers, gunners, and cannons to defend against waves of attacking ninjas. Players can promote their units and utilize items like smoke bombs and rice balls to enhance their defense.

The storyline of Dairojo! Samurai Defenders revolves around the fierce battles between Shoguns as they vie for control over Japan, with iconic castles under siege. Players must navigate the complexities of warfare while facing deceptive tactics, exemplified by the character Hana, the Geisha.

Dairojo! Samurai Defenders stands out with its multiple gameplay modes, including Score Attack, Normal Mode, Random Mode, and Versus, along with a "Fast Forward" function and various difficulty levels, offering endless gameplay possibilities.