In Cities in Motion 2, players take on the role of a transport company director, tasked with constructing and managing profitable transport networks across various cities. The game allows players to create routes, select vehicle models, manage employee salaries, and set fares, all while considering the needs and preferences of different passenger classes.

The storyline revolves around the challenge of efficiently connecting residents to their workplaces and essential services through a variety of transport modes, including buses, trams, metros, river trams, and trolleybuses.

Cities in Motion 2 stands out with its detailed simulation of urban transport dynamics, the introduction of a day-night cycle, and the ability to build roads. Additionally, the game features a sandbox mode for unlimited resource management and a campaign mode with specific objectives tied to different cities.