In Assassin's Creed Brotherhood, players control Ezio Auditore, who can recruit and command a Brotherhood of Assassins while utilizing a variety of weapons and tools to eliminate enemies. The game features a mix of single-player and multiplayer modes, allowing players to engage in both story-driven missions and competitive gameplay.

The storyline of Assassin's Creed Brotherhood follows Ezio as he seeks to defeat the corrupt Borgia family in Renaissance Rome, aiming to restore freedom to the city and strengthen the Assassin Brotherhood. Players navigate through a richly detailed open world, completing missions and building alliances.

Assassin's Creed Brotherhood stands out with its innovative multiplayer component, which allows players to choose from various character classes, each with unique abilities. The game also introduces a guild system, enabling players to recruit and train their own Assassins, adding depth to the gameplay experience.